Luminex xPONENT for MAGPIX Instrukcja Użytkownika Pobierz pdf (Strona 110)

Plate Name - The name assigned to the plate.

CAUTION: If using multiple plates users must be careful to ensure that

plates are used in the proper order. Failure to do so can result in

inaccurate data and test results.

Commands - This section contains the following commands:

• Wash - Sends 250µl distilled water through the chamber and fluidic lines in the system. It

pulls the fluid from a well or from the reservoir and runs it completely through the system to

the waste receptacle.

• Soak - Prevents clogs in the probe, chamber, and sample loop. This command uses 200µl

of DI water from an off-plate reservoir; the system expels fluid into the system waste

container.

• Rinse - Re-primes the probe and rinses out the chamber. You do not have to add any

reagent. One of the off-plate reservoirs must be empty because the system will expel fluid

into the reservoir.

• CAL - Calibrates the LEDs and focuses the camera.

NOTE: When you choose the CAL command, a Rinse command is

performed prior to the CAL. When the CAL is complete, two

additional Rinse commands are performed. This prevents a bubble

from loading into the chamber. Verify that you have sufficient room in

the reservoir to complete these Rinse commands.

• Alcohol Flush - Removes air bubbles from the fluidics line and chamber using 70%

isopropanol or 70% ethanol. This command uses 100µl of alcohol from the off-plate

reagent block reservoir.

• Prime - Removes air from the system’s fluidic pathways by drawing Drive Fluid from the

Drive Fluid container. You do not need to supply solution in a plate.

• VER - Verifies the system is performing to specifications.

NOTE: When you choose the VER command, a Rinse command is

performed prior to the VER. When the VER is complete, an

additional Rinse command is performed. This prevents a bubble

from loading into the chamber. Verify that you have sufficient room in

the reservoir to complete these Rinse commands.

• Sanitize - The sanitize command performs a similar function as the alcohol flush

command, but uses 100µl of 10% to 20% household bleach and water solution to

decontaminate sample lines after biohazard contact. Sanitize as part of your daily

shutdown routine after biohazard contact.

• Self Test - Performs a self-diagnostic to see if the system and all operations are

functioning correctly.

NOTE: You should perform a Self Test as part of your scheduled weekly

maintenance.

Fluidics - Runs fluidics to check well-to-well carryover.

Performing System Maintenance

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Luminex xPONENT for MAGPIX Instrukcja Użytkownika Strona 110