Luminex 100 IS Developer Workbench Guide Version 2.3 Instrukcja Użytkownika Strona 64
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Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology
58 PN 89-00002-00-084 Rev. B
Enumerate the Coupled
Microspheres
1. Dilute the resuspended coupled microspheres 1:100 in deionized
water.
2. Mix thoroughly using a vortex.
3. Transfer 10 µL to a hemacytometer.
4. Count the microspheres within the 4 large corners of the
hemacytometer grid.
5. Microspheres/µL = (Sum of microspheres in 4 large corners) x
2.5 x 100 (dilution factor).
6. Store the preparation at 2
o
C - 8°C. Protect it from light.
Technical Notes 1. Synthesize the Oligonucleotides with a 5’ or 3’ amino group and
spacer modification. No Tris or Azide or other amine-containing
buffers should be present during the coupling procedure.
Preferably, resuspend the oligonucleotide in water following
synthesis. If oligonucleotides were previously solubilized in an
amine-containing buffer, then precipitation and resuspension into
water. Refer to http://luminexcorp.custhelp.com for more
information.
2. You can scale this procedure up or down. Refer to http://
luminexcorp.custhelp.com for more information.
3. The optimal coupling concentration for a given oligonucleotide
is determined by coupling at various concentrations within the
recommended range of 1 to 100 µM.
4. Minimize the exposure of EDC to air; secure closures on stock
and dry aliquot containers, store desiccated at -20°C. Use
aliquots immediately and discard containers after use. Make a
fresh 10 mg/mL EDC solution before each addition.
5. For best results, use microcentrifuge tubes from USA Scientific,
Inc., catalog number 1415-2500. To order, call 1 800 LAB-TIPS.
- Luminex 100 1
- IS Developer 1
- Workbench Guide Version 2.3 1
- PN 89-00002-00-084 Rev. B 2
- August, 2005 2
- Contents 3
- A xMAP Protocols 49 4
- Version 1.7 61 5
- C Customize Splash Screen 63 5
- Index 65 5
- Technology 6
- Developer Workbench Guide 7
- General Guidelines 8
- Instructions 9
- Instrument Calibrators 12
- Developer 13
- Workbench 13
- Software 13
- Procedures 14
- Dialog Box Buttons 15
- Create New Product 15
- 10 PN 89-00002-00-084 Rev. B 16
- PN 89-00002-00-084 Rev. B 11 17
- 12 PN 89-00002-00-084 Rev. B 18
- PN 89-00002-00-084 Rev. B 13 19
- 14 PN 89-00002-00-084 Rev. B 20
- Quantitative Assay 21
- Template 21
- 16 PN 89-00002-00-084 Rev. B 22
- PN 89-00002-00-084 Rev. B 17 23
- Note: You can use the same 23
- 18 PN 89-00002-00-084 Rev. B 24
- Figure 7. Select Bead IDs 24
- PN 89-00002-00-084 Rev. B 19 25
- Qualitative Assay 27
- 22 PN 89-00002-00-084 Rev. B 28
- PN 89-00002-00-084 Rev. B 23 29
- 24 PN 89-00002-00-084 Rev. B 30
- PN 89-00002-00-084 Rev. B 25 31
- Figure 13. Select Bead IDs 31
- 26 PN 89-00002-00-084 Rev. B 32
- PN 89-00002-00-084 Rev. B 27 33
- PN 89-00002-00-084 Rev. B 29 35
- 30 PN 89-00002-00-084 Rev. B 36
- PN 89-00002-00-084 Rev. B 31 37
- 32 PN 89-00002-00-084 Rev. B 38
- Figure 21. Select Bead IDs 38
- PN 89-00002-00-084 Rev. B 33 39
- 34 PN 89-00002-00-084 Rev. B 40
- Maintenance 41
- Edit an Unused 42
- Edit a Used 43
- To edit a used template: 43
- Delete an Unused 44
- Delete a Used 45
- To delete a used template: 45
- Print Template 46
- Information 46
- PN 89-00002-00-084 Rev. B 41 47
- Technology 48
- To import a template: 49
- To export a template: 50
- About Developer 51
- Luminex Technical 53
- Support Website 53
- Handling 55
- Enumeration of xMAP 56
- Microsphere Suspensions 56
- PN 89-00002-00-084 Rev. B 51 57
- Protein Coupling Protocol 58
- Procedure 59
- Coupling 60
- Blocking, and 60
- PN 89-00002-00-084 Rev. B 55 61
- Equipment •Vortex 62
- PN 89-00002-00-084 Rev. B 57 63
- Enumerate the Coupled 64
- Microspheres 64
- LumAvidin Binding Protocol 65
- 60 PN 89-00002-00-084 Rev. B 66
- Version 1.7 67
- 62 PN 89-00002-00-084 Rev. B 68
- Customize Splash Screen 69
- 64 PN 89-00002-00-084 Rev. B 70
- PN 89-00002-00-084 Rev. B 65 71
- 66 PN 89-00002-00-084 Rev. B 72
- PN 89-00002-00-084 Rev. B 67 73
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