Luminex 200 User Manual with LDS 1.7 Software Instrukcja Użytkownika Strona 1

Luminex 200™ User Manual for LDS Version 1.7 Lum/nex ®

iv PN 89-00002-00-150 Rev. ALuminex 200 User Manual for LDS Version 1.7 xMAP TechnologyBead Detail Irregularities . . . . . . . . . . . . . . . . . .

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology7 - 12 PN 89-00002-00-150 Rev. A

Luminex 200 Maintenance Log Month(s): ________________________________________Year:_______________Procedure: To use this lo

PN 89-00002-00-150 Rev. A 8 - 1TroubleshootingTroubleshooting procedures help users isolate, identify, and remedy problems with the Luminex analyzer a

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology8 - 2 PN 89-00002-00-150 Rev. AFor assistance, contact Luminex Technical Support via email.

xMAP Technology TroubleshootingPN 89-00002-00-150 Rev. A 8 - 3• Instrument control, sample acquisition, session uploading, and start, stop and pause f

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology8 - 4 PN 89-00002-00-150 Rev. AFluid Leaks Fluid leaks can result in poor pressurization an

xMAP Technology TroubleshootingPN 89-00002-00-150 Rev. A 8 - 5Sample Probe Problems with the sample probe can lead to fluid leaks and pressurization p

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology8 - 6 PN 89-00002-00-150 Rev. ASample arm stuck in the down positionThe sample probe height

xMAP Technology TroubleshootingPN 89-00002-00-150 Rev. A 8 - 7Calibration ProblemsThe sample probe is bent. Remove the sample probe from the Luminex a

PN 89-00002-00-150 Rev. A vxMAP Technology ContentsLuminex Sheath Delivery System C-1Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology8 - 8 PN 89-00002-00-150 Rev. AThe calibration microspheres are not fully suspended.Vortex

xMAP Technology TroubleshootingPN 89-00002-00-150 Rev. A 8 - 9Acquisition ProblemsBeads were left in the system after Classification calibration.Perfo

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology8 - 10 PN 89-00002-00-150 Rev. AThe wrong wells are selected in the Setup XY tab.On the Opt

xMAP Technology TroubleshootingPN 89-00002-00-150 Rev. A 8 - 11Bead Detail Irregularities You can diagnose many problems by using the histogram descri

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology8 - 12 PN 89-00002-00-150 Rev. ACalibration microspheres classify too highYou may be using

xMAP Technology TroubleshootingPN 89-00002-00-150 Rev. A 8 - 13Error MessagesError Type Possible Cause SolutionUnable to calculate temperature compens

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology8 - 14 PN 89-00002-00-150 Rev. APrinting ErrorsVerificationPerform the verification procedu

xMAP Technology TroubleshootingPN 89-00002-00-150 Rev. A 8 - 151. Click Warmup on the toolbar. Wait 30 minutes for the warmup to complete.2. On the An

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology8 - 16 PN 89-00002-00-150 Rev. Afrom the Luminex 200 system, copy the files to a floppy dis

PN 89-00002-00-150 Rev. A 9 - 1ProtocolsUpdates or additions to these protocols are posted on the Luminex website at http://www.luminexcorp.com.The pr

vi PN 89-00002-00-150 Rev. ALuminex 200 User Manual for LDS Version 1.7 xMAP Technology

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology9 - 2 PN 89-00002-00-150 Rev. A3. Sonicate the vial in a water bath sonicator to disperse t

xMAP Technology ProtocolsPN 89-00002-00-150 Rev. A 9 - 3Enumeration of xMAP Microsphere SuspensionsLuminex xMAP microspheres are provided at standard

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology9 - 4 PN 89-00002-00-150 Rev. AxMAP Microsphere Stability and StoragexMAP microspheres are

xMAP Technology ProtocolsPN 89-00002-00-150 Rev. A 9 - 5• Sulfo-NHS: N-Hydroxysulfosuccinimide sodium salt, Pierce Chemicals•EDC: 1-Ethyl-3-(3-dimethy

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology9 - 6 PN 89-00002-00-150 Rev. A11. Incubate the suspension for 20 ± 2 minutes in the dark a

xMAP Technology ProtocolsPN 89-00002-00-150 Rev. A 9 - 7amines. If any of these agents exist in the protein preparation, remove them through dialysis

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology9 - 8 PN 89-00002-00-150 Rev. A•SDS:Sodium Dodecyl Sulfate (0.1% w/v)Preparation 1. Allow a

xMAP Technology ProtocolsPN 89-00002-00-150 Rev. A 9 - 916. Centrifuge the xMAP microspheres at 8,000 × g or higher for 1 minute.17. Remove the supern

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology9 - 10 PN 89-00002-00-150 Rev. A• Micropipetters (1 µl - 1000 µl)• Microcentrifuge•Timer•Ro

xMAP Technology ProtocolsPN 89-00002-00-150 Rev. A 9 - 119. Add 500 µl of BLOCKING/STORAGE BUFFER to the microspheres and vortex. Centrifuge the tube

PN 89-00002-00-150 Rev. A 1 - 1IntroductionThe Luminex® 200™ system is a compact analysis unit consisting of a Luminex analyzer, the Luminex XY Platfo

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology9 - 12 PN 89-00002-00-150 Rev. A

PN 89-00002-00-150 Rev. A A - 1Glossaryaggregated microspheresTwo or more xMAP microspheres that have adhered together.Alexa 532 Fluorochrome used for

A - 2 PN 89-00002-00-150 Rev. ALuminex 200 User Manual for LDS Version 1.7 xMAP Technologycalibrators xMAP microspheres used to normalize the settings

PN 89-00002-00-150 Rev. A A - 3xMAP Technology emission spectrumemission spectrum Wavelength range that an excited fluorochrome emits when its electro

A - 4 PN 89-00002-00-150 Rev. ALuminex 200 User Manual for LDS Version 1.7 xMAP TechnologyxMAP microsphere set Luminex multi-analyte microspheres cont

PN 89-00002-00-150 Rev. A A - 5xMAP Technology reporterreporter A molecule (or combination of molecules) with a specific range of excitation and emiss

A - 6 PN 89-00002-00-150 Rev. ALuminex 200 User Manual for LDS Version 1.7 xMAP Technology

PN 89-00002-00-150 Rev. A B - 1Product NumbersNote: These part numbers are subject to change without notification.HardwareBAir filter (accessed throug

B - 2 PN 89-00002-00-150 Rev. ALuminex 200 User Manual for LDS Version 1.7 xMAP TechnologySoftwareReagentsPower cord CN-P002-01 (Australia)CN-P003-01

PN 89-00002-00-150 Rev. A B - 3xMAP TechnologyTrainingxMAP development microspheres (carboxylated)1, 4, and 16 mLL100-CDEV1-[size code of 01, 04, or 1

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology1 - 2 PN 89-00002-00-150 Rev. Aits own subset, based on its fluorescent signature. In addit

B - 4 PN 89-00002-00-150 Rev. ALuminex 200 User Manual for LDS Version 1.7 xMAP Technology

PN 89-00002-00-150 Rev. A C - 1Luminex Sheath Delivery SystemOverview Use this appendix to install, use, and maintain the Luminex Sheath Delivery Syst

Luminex 200 User Manual for LDS Version 1.7 xMAP TechnologyC - 2 PN 89-00002-00-150 Rev. AWARRANTY RIGHTS PROVIDED BY LUMINEX AND/OR ITS AUTHORIZED PA

xMAP Technology Luminex Sheath Delivery SystemPN 89-00002-00-150 Rev. A C - 3Sheath Delivery System SetupWhen adjusting the Luminex 200 regulator, tur

Luminex 200 User Manual for LDS Version 1.7 xMAP TechnologyC - 4 PN 89-00002-00-150 Rev. A• Connect the other end of the air line to the air connector

xMAP Technology Luminex Sheath Delivery SystemPN 89-00002-00-150 Rev. A C - 510. In the Luminex Data Collector Main window, click Prime. During this p

Luminex 200 User Manual for LDS Version 1.7 xMAP TechnologyC - 6 PN 89-00002-00-150 Rev. AMaintenance and CleaningPerform proper maintenance and clean

xMAP Technology Luminex Sheath Delivery SystemPN 89-00002-00-150 Rev. A C - 7Filter. If the filter attached to the sheath intake line becomes clogged

Luminex 200 User Manual for LDS Version 1.7 xMAP TechnologyC - 8 PN 89-00002-00-150 Rev. ALabels The following labels are attached to the back of the

PN 89-00002-00-150 Rev. A Index-1Numerics100 region bead map 6-225 region bead map 6-250 region bead map 6-2Aaccess doors 3-8accuracy/precisionspecifi

xMAP Technology IntroductionPN 89-00002-00-150 Rev. A 1 - 3container and effectively removes residual sample within the tubing, valves, and probe. The

Index-2 PN 89-00002-00-150 Rev. ALuminex 200 User Manual for LDS Version 1.7 xMAP Technologycaution 2-6cautions 2-2, 2-5, 2-8CE mark 2-4centrifugation

PN 89-00002-00-150 Rev. A Index-3xMAP Technology Indexheater temperature 6-22height adjustment locking screw 4-4high voltage 6-22, 6-24histogram 6-17b

Index-4 PN 89-00002-00-150 Rev. ALuminex 200 User Manual for LDS Version 1.7 xMAP Technologyspecifications 3-4options dialog box 6-1PPC specifications

PN 89-00002-00-150 Rev. A Index-5xMAP Technology Indexaccuracy/precision 3-2capacity 3-3classification laser 3-4electronics 3-4fluidics 3-4Luminex 100

Index-6 PN 89-00002-00-150 Rev. ALuminex 200 User Manual for LDS Version 1.7 xMAP Technology

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology1 - 4 PN 89-00002-00-150 Rev. AFluorescence CompensationFluorescent spillover occurs when a

xMAP Technology IntroductionPN 89-00002-00-150 Rev. A 1 - 5from the main menu bar, from the toolbar, and from menus that appear when you right-click a

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology1 - 6 PN 89-00002-00-150 Rev. A

PN 89-00002-00-150 Rev. A 2 - 1SafetyYour safety is important. Please do not perform procedures on your Luminex 200 system that are not specifically c

© LUMINEX CORPORATION, 1998 - 2005. All rights reserved. No part of this publication may be reproduced, transmitted, transcribed, or translated into a

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology2 - 2 PN 89-00002-00-150 Rev. ASafety and Regulatory SymbolsThese symbols describe warnings

xMAP Technology SafetyPN 89-00002-00-150 Rev. A 2 - 3Danger: This message is used in cases where significant risk of serious injury or death is presen

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology2 - 4 PN 89-00002-00-150 Rev. AFigure 2-2. European Safety Requirements LabelThe Luminex a

xMAP Technology SafetyPN 89-00002-00-150 Rev. A 2 - 5Fluidics The Luminex analyzer contains fluidics. In the event of a fluid leak, turn off all power

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology2 - 6 PN 89-00002-00-150 Rev. AFigure 2-6. Laser Caution LabelDo not remove the analyzer c

xMAP Technology SafetyPN 89-00002-00-150 Rev. A 2 - 7Figure 2-8. Avoid Exposure LabelMechanicalBiologicalCaution: Use of controls or adjustments or p

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology2 - 8 PN 89-00002-00-150 Rev. AHeatBlue Indicator LightThe blue light above the sample arm

PN 89-00002-00-150 Rev. A 3 - 1System OverviewHardware The Luminex 200 for LDS Version 1.7 system includes the following hardware:• Luminex analyzer•

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology3 - 2 PN 89-00002-00-150 Rev. ARequired Laboratory ReagentsThe following items are necessar

xMAP Technology System OverviewPN 89-00002-00-150 Rev. A 3 - 3Sensitivity • Detect 1000 fluorochromes phycoerythrin (PE) per xMAP microsphere• Reporte

Standard Terms and Conditions For Use of ProductBy opening the packaging containing this product ("Product") or by using such Product in any

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology3 - 4 PN 89-00002-00-150 Rev. A• AC inlet fuse: 3 Amp, 250 V~, fast actingOptics • Reporter

xMAP Technology System OverviewPN 89-00002-00-150 Rev. A 3 - 5Luminex SD System General• Ambient temperature: 15°C to 30°C (59° to 86°F)• Humidity: 20

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology3 - 6 PN 89-00002-00-150 Rev. Aand be UL listed, CSA certified, CE marked for nondomestic u

xMAP Technology System OverviewPN 89-00002-00-150 Rev. A 3 - 7Luminex XYPInstrumentVentilation FilterThe XYP instrument ventilation filter cleans the

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology3 - 8 PN 89-00002-00-150 Rev. AFluidics Luminex XYP Sample Probe: A stainless steel sampl

xMAP Technology System OverviewPN 89-00002-00-150 Rev. A 3 - 9Figure 3-3 Front View of Luminex analyzer Showing Access DoorsAir filter and access doo

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology3 - 10 PN 89-00002-00-150 Rev. ASyringe: The syringe delivers a sample from the 96 well mi

xMAP Technology System OverviewPN 89-00002-00-150 Rev. A 3 - 11Figure 3-7 Left Side of Analyzer - Air and Fluid ConnectorsSheath fluid container (not

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology3 - 12 PN 89-00002-00-150 Rev. AOptical The optical system contains the optics assembly and

PN 89-00002-00-150 Rev. A 4 - 1InstallationLuminex 200 System InstallationEnsure that the facility complies with all system and safety requirements. I

strict compliance with the United States Food and Drug Administration and all applicable domestic and international laws and regulations, now and here

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology4 - 2 PN 89-00002-00-150 Rev. A5. Place the Luminex analyzer on top of the Luminex XYP. Do

xMAP Technology InstallationPN 89-00002-00-150 Rev. A 4 - 3Luminex 200 Software SetupAfter performing the Luminex 200 system assembly, ensure that the

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology4 - 4 PN 89-00002-00-150 Rev. AAdjusting the Sample Probe HeightYou must adjust the sample

xMAP Technology InstallationPN 89-00002-00-150 Rev. A 4 - 5For a filter bottom plate—stack three of the larger (5.08 mm diameter) alignment discs toge

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology4 - 6 PN 89-00002-00-150 Rev. AInstalling Software and FirmwareThe Luminex 200 system arriv

xMAP Technology InstallationPN 89-00002-00-150 Rev. A 4 - 7Time, Monday through Friday. Users outside of the U.S. and Canada can call us at +1 512-381

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology4 - 8 PN 89-00002-00-150 Rev. A

PN 89-00002-00-150 Rev. A 5 - 1 System StartupThis chapter describes how to start up your system. The topics included are:• Luminex Main Window• Calib

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology5 - 2 PN 89-00002-00-150 Rev. AThe Luminex Main window has six components: Title bar, Menu

xMAP Technology System StartupPN 89-00002-00-150 Rev. A 5 - 3 To add a new lot of xMAP classification calibration microspheres:1. Click Calibrate. T

End-User License Agreement (EULA) for Luminex® SoftwareThis Luminex End-User License Agreement (“EULA”) is a legal agreement between you (either an in

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology5 - 4 PN 89-00002-00-150 Rev. AAdd New Reporter Channel Calibration LotsBefore you perform

xMAP Technology System StartupPN 89-00002-00-150 Rev. A 5 - 5Calibrate the Luminex AnalyzerIn addition to calibrating the analyzer after installation,

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology5 - 6 PN 89-00002-00-150 Rev. A7. Fill the reservoir with a solution of 70% isopropanol or

xMAP Technology System StartupPN 89-00002-00-150 Rev. A 5 - 7The progress bar shows the calibration progress, which should take less than two minutes.

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology5 - 8 PN 89-00002-00-150 Rev. AFigure 5-9. Print Dialog Box3. Check the box in front of th

xMAP Technology System StartupPN 89-00002-00-150 Rev. A 5 - 9more) in the gain value for a channel from one calibration to the next, the difference ma

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology5 - 10 PN 89-00002-00-150 Rev. Aif a Luminex XYP instrument is added to the system. See pag

xMAP Technology System StartupPN 89-00002-00-150 Rev. A 5 - 11recommend that you do not cancel warmup. Your test results will be accurate only if the

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology5 - 12 PN 89-00002-00-150 Rev. A

PN 89-00002-00-150 Rev. A 6 - 1Data AcquisitionThe Luminex analyzer uses Luminex Data Collector software to acquire data. This chapter describes the f

be accessed through use of the SOFTWARE PRODUCT is the property of the respective content owner and may be protected by applicable copyright or other

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 2 PN 89-00002-00-150 Rev. ASound effects: Enables sounds during system operation.Clear

xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 3Acquisition tab On this tab you can select the default folder for your session files. I

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 4 PN 89-00002-00-150 Rev. AShow APD Voltages. This is a Field Service option. Do not se

xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 5To use the heater, check Enabled in the Heater area. Use the Set Point control to selec

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 6 PN 89-00002-00-150 Rev. ACreate a Session Using Default Settings To create a sessio

xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 72. Select the template that you want to use for this session and click OK. If the templ

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 8 PN 89-00002-00-150 Rev. AOpen and Add to an Existing Session To open an existing ses

xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 93. Use the settings shown or modify them, then highlight the first vacant sample.4. Cli

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 10 PN 89-00002-00-150 Rev. A2. Make desired setting changes. See “Session Settings” on

xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 11Open. Opens an existing sessionClose Session. Closes the current session. Prompts you

PN 89-00002-00-150 Rev. A iContentsIntroduction 1-1Principle of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1Basi

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 12 PN 89-00002-00-150 Rev. AHelp Contents. Opens the online help.Search for Help On. Op

xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 13Clear All: Clears the screen of all data and graphs. This function deletes all associ

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 14 PN 89-00002-00-150 Rev. ARight-Click Menu Other commands appear on the right-click m

xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 15We recommend using at least 100 events per microsphere set. This guarantees that each

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 16 PN 89-00002-00-150 Rev. ABead Set tab On the Bead Set tab you can select and name th

xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 17Histogram The histogram is located in the lower-left corner of the screen. The histogr

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 18 PN 89-00002-00-150 Rev. A2. Click X axis, then click on the desired parameter name.I

xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 19Use the Show Bead options to set the histogram to show events for only one bead set, a

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 20 PN 89-00002-00-150 Rev. Afor later assays or sessions. By increasing this voltage se

xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 21density is indicated by contrasting colors. A decaying dot plot shows the 100 most rec

ii PN 89-00002-00-150 Rev. ALuminex 200 User Manual for LDS Version 1.7 xMAP TechnologyLuminex XYP Specifications. . . . . . . . . . . . . . . . . . .

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 22 PN 89-00002-00-150 Rev. Athe beads are hitting with respect to their region. The bea

xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 23The message at the top reflects the current status of the Luminex analyzer.Ready: Indi

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 24 PN 89-00002-00-150 Rev. AThe system monitor section also shows information about tem

xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 255. In the results table of the new session, highlight the sample where you want to beg

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 26 PN 89-00002-00-150 Rev. A9. Remove the microtiter plate from the plate holder.10. Cl

xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 279. Remove the microtiter plate from the plate holder.10. Click Retract from the toolba

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 28 PN 89-00002-00-150 Rev. A To interrupt sample acquisition between sample runs:1. Cl

xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 296. In the Manufacturers List, click Generic. In Printers list, click Generic/Text Only

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 30 PN 89-00002-00-150 Rev. A

PN 89-00002-00-150 Rev. A 7 - 1Maintenance and CleaningProper maintenance and cleaning of the Luminex analyzer should be performed in order to preserv

PN 89-00002-00-150 Rev. A iiixMAP Technology ContentsDelete a Template. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-10Multiplex

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology7 - 2 PN 89-00002-00-150 Rev. AStartup  Perform the entire startup procedure on a daily ba

xMAP Technology Maintenance and CleaningPN 89-00002-00-150 Rev. A 7 - 3Check Fluid Levels Check the sheath and waste fluids daily, more often if you r

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology7 - 4 PN 89-00002-00-150 Rev. AClean Accessible Surfaces Clean the exterior surfaces of th

xMAP Technology Maintenance and CleaningPN 89-00002-00-150 Rev. A 7 - 5Replace Air Intake FiltersYou should replace the filters on the Luminex analyze

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology7 - 6 PN 89-00002-00-150 Rev. A Figure 7-2. Back of Luminex XYP, Removing Air Filter3. Rep

xMAP Technology Maintenance and CleaningPN 89-00002-00-150 Rev. A 7 - 71. Syringe Seal 2. SyringeFigure 7-3. Front of Luminex 200 Analyzer,

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology7 - 8 PN 89-00002-00-150 Rev. A3. Connect the new sheath filter, matching up the color-code

xMAP Technology Maintenance and CleaningPN 89-00002-00-150 Rev. A 7 - 9Change Fuses  To replace fuses on the Luminex analyzer or the Luminex XYP ins

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology7 - 10 PN 89-00002-00-150 Rev. AStoring the Analyzer  To prepare the system for storage:1.

xMAP Technology Maintenance and CleaningPN 89-00002-00-150 Rev. A 7 - 11Decontaminating the Luminex Analyzer for Return ShipmentLuminex Technical Supp