Luminex FLEXMAP 3D User Manual Instrukcja Użytkownika

Luminex® FLEXMAP 3D®Hardware User ManualFor research use only. Not for use indiagnostic procedures.

Luminex® FLEXMAP 3D® Hardware User ManualxFor research use only. Not for usein diagnostic procedures.

Become familiar with the information in this chapter before using the equipment. Do notperform procedures on your system that are not specifically con

SymbolsYou may encounter these symbols during the use and operation of the Luminex® FLEXMAP3D® system. They represent warnings, conditions, identifica

FIGURE 1.MET MarkIn addition, Luminex FLEXMAP 3D complies with European Union (EU) safety requirementsand therefore may be marketed in the Europe Sing

WARNING: Do not use this device in close proximity to sources of strongelectromagnetic radiation, for example, unshielded intentionalRF sources, as th

of procedures other than those specified in this manual can resultin hazardous radiation exposure.The bar code reader laser presents a potential hazar

Mechanical ComponentsWARNING: The Luminex® FLEXMAP 3D® instrument has parts that moveduring operation.Risk of personal injury is present. The moving p

FIGURE 5.Fuse Caution LabelThe following voltage label appears on the back of the FLEXMAP 3D® instrument. It displaysthe FLEXMAP 3D® serial number, mo

WARNING: Wear appropriate personal protective equipment when handlingparts that come into contact with potentially biohazardoussamples.To decontaminat

Disposal of InstrumentNOTE: Within the European Union and other jurisdictions, in accordancewith Directive 2002/96/EC of the European Parliament and o

© Luminex Corporation, 2012. All rights reserved. No part of this publication may bereproduced, transmitted, transcribed, or translated into any langu

Luminex® FLEXMAP 3D® Hardware User Manual10For research use only. Not for usein diagnostic procedures.

This chapter reviews the technical aspects of the Luminex® FLEXMAP 3D® system:operation, system components, subsystems, recommended additional equipme

SubsystemsThe Luminex® FLEXMAP 3D® instrument includes four subsystems: electronic, fluidic,mechanical, and optical. The following topics describe the

FIGURE 8.Back of the FLEXMAP 3D® Instrument1 Power Input Module2 Communications Port P1 (USB type B)FluidicsThe fluidics system handles the flow of li

FIGURE 9.Front of the FLEXMAP 3D® Instrument1 Left door, with access to sheath filter and door ventilation filter2 Right door, with access to syringe

FIGURE 10.Sample Probe1 Cheminert fitting2 Attachment point on Cheminert fitting for sample tubing3 Sample probe, in up positionSyringe PumpThe syring

FIGURE 11.Syringe Pump1 Syringe arm (within dotted lines)2 Glass syringe barrel3 Syringe plungerSheath FilterThe sheath filter removes particles great

FIGURE 12.Fluidics Bay1 Sheath filter2 Blue tubing attached to sheath fluid container3 Orange tubing attached to waste fluid containerWaste Fluid Cont

of this manual. The "Fluidics Bay" image above and "Front Door Ventilation Filters" imagebelow show the location of these filters.

2 Holding clamp3 Cover over HEPA air filterOpticsThe optical system consists of the optical assembly and the excitation lasers. The opticalcomponents

Standard Terms and Conditions for Use of InstrumentProductBy opening the packaging containing this instrument product ("Product") or by usin

CAUTION: Adhere to standard laboratory safety practices when handlinghazardous, toxic, or flammable reagents and chemicals. ContactLuminex Technical S

CAUTION: Luminex calibrators and verifiers contain sodium azide. Sodiumazide is toxic and may also react with lead and copper plumbingto form highly e

PrinterUse a printer compatible with Microsoft® Windows XP SP3.Barcode LabelsUse Code 128 barcode label type when scanning barcode labels into the sys

Temperature control: Maintains samples using the heater block at a constant temperature from 35°C to 60°C(95°F to 131°F), +/- 1°C of setpoint.Altitude

ElectronicsUSB 2.0-compatible communications link for fast data transferInput voltage range: 100-120 V~, 6.0 A, 50/60 Hz or, 200-240 V~, 3.0 A, 50-60

Detects and distinguishes surface reporter fluorescence emissions at 575 nm on the surface of 1 to500 unique xMAP microspheres in a single sampleSolub

Luminex® FLEXMAP 3D® Hardware User Manual26For research use only. Not for usein diagnostic procedures.

To ensure accurate test results, properly clean and maintain the Luminex® FLEXMAP 3D®system. Read and follow all instructions in this section. Perform

the performance of these activities, see the appropriate Luminexsoftware manual or Luminex online help.Initializing the Luminex® FLEXMAP 3D® Instrumen

WARNING: Do not move the waste line vertically while the FLEXMAP 3D®instrument is running.Contact Luminex Technical Support before relocating the wast

hardware not provided by Luminex. If Product is purchased from a Luminex authorizedreseller, any warranty obligations shall be provided in writing dir

Removing ClogsNOTE: If you frequently use the Luminex® FLEXMAP 3D® system to testconcentrated serum or other debris ridden samples, Luminexrecommends

4. Replace the sample probe and tightly screw in the Cheminert fitting.5.Use the software to perform an automatic probe height adjustment. NOTE: Perfo

Monthly MaintenanceClean the exterior surfaces monthly.To clean exterior surfaces:1.Turn off the Luminex® FLEXMAP 3D® instrument and unplug the power

9. Screw the syringe back into its housing.10.Return the syringe arm to its original position. The bottom of the plunger fits into theindentation in t

Replacing the HEPA Air FilterThe HEPA air filter is the round filter behind the panel on the left side of the front of thefluidics bay. Tubing from th

Cleaning the Ventilation FiltersEach filter has at least one imprinted arrow on its metal frame. This arrow indicates thedirection of the air flow. Fi

8. Reinstall the XY cover and close the access doors.9.Plug in the power cord and turn on the FLEXMAP 3D® instrument. FIGURE 18.Ventilation Filter on

View A Right front door, viewed from inside 2 Filter ClampView B Left front door, viewed from inside 3 Incised Arrow1 FilterAnnual MaintenanceAnnual m

6. Plug in the power cord and turn on the FLEXMAP 3D® instrument.7.Use the software to run the prime command twice. FIGURE 20.Front of Fluidics Bay (I

2. Remove the sample probe from the instrument and flush it with deionized water from thenarrow end out through the larger end.3.Replace the sample pr

Luminex's laser based fluorescent analytical test instrumentation. Buyer furtheracknowledges that the Product has not received approval from the

4. Check both of the fuses in the cartridge for damage. A fuse can display physicalevidence of damage, for example, broken wire or blackened glass; if

maintenance (one task monthly), semi-annual maintenance (two tasks twice yearly), andannual maintenance (two tasks yearly). For each item listed at th

Luminex® FLEXMAP 3D® Hardware User Manual42For research use only. Not for usein diagnostic procedures.

Troubleshooting procedures help users identify and remedy problems with the instrument.OverviewTo troubleshoot a problem, locate the symptom in one of

Power Supply ProblemsPower supply problems often involve a blown fuse, faulty electronic component, ordisconnected cable.CAUTION: Whenever you deal wi

ClogsOften, a clog somewhere in the Luminex® FLEXMAP 3D® instrument is the cause of a fluidleak, a pressurization or sample probe problem, or a proble

Fluid LeaksFluid leaks can result in poor pressurization and failed sample acquisition.TABLE 4.Fluid LeaksSymptom Possible cause SolutionFluid is pool

Symptom Possible cause SolutionThe microtiter plate is warped. Inspect the microtiter plate. Replace it if itis warped.The sample probe is bent. Remov

Symptom Possible cause SolutionThere is a problem internal to theinstrument.Review the log of calibration reports.Check for dramatic changes intempera

Symptom Possible cause SolutionThe waste line was moved duringinstrument operation, resulting in anunstable flow rate.Stabilize the waste line during

consulting fees) that any of the Released Parties may sustain or incur as a result of any claimagainst such Released Party based upon negligence, brea

Symptom Possible cause SolutionThe microspheres are photobleached. Replace the microspheres withunphotobleached microspheres.There are an insufficient

TABLE 9.Bead Detail IrregularitiesSymptom Description Possible Problem SolutionxMAP and calibrator microspheres form a group in the wrong location.Cal

Symptom Description Possible Problem SolutionxMAP microspheres do notform a cohesive group and aresomewhat scattered.There is air in the instrument. V

ShippingIf a serious problem arises with the Luminex® FLEXMAP 3D® system, it may be necessary toreturn it to Luminex Corporation for repairs. If Lumin

6. Connect the waste fluid tubing (orange connector) to the bottom quick disconnect point(white connection) of the sheath filter tubing.7.Use the soft

YesNoPrinted Name _____________________________________________________Signature ________________________________________________________Company/Insti

Luminex® FLEXMAP 3D® Hardware User Manual56For research use only. Not for usein diagnostic procedures.

Installation of the Luminex® FLEXMAP 3D®The following installation instructions for Luminex® FLEXMAP 3D® are to be used for siteprep, assessing facili

2. Connect the FLEXMAP 3D® instrument and all necessary peripheral devices to the PC. FIGURE 24.Back of PC1. Monitor Plug 5. Monitor Plug (Touchscreen

1. Power Inlet 2. PC Communications plug (USBtype B)3. Connect the USB communications cable between the FLEXMAP 3D® instrument (P1)and the PC, and con

Table of ContentsChapter 1 Safety ...1D

18. Calibrate the probe height using the instructions in the software user manual. NOTE: For information on calibrating the probe height when using so

For research use only. Not for use in diagnostic procedures.Installation61

Luminex® FLEXMAP 3D® Hardware User Manual62For research use only. Not for usein diagnostic procedures.

For research use only. Not for use in diagnostic procedures.Installation63

Luminex® FLEXMAP 3D® Hardware User Manual64For research use only. Not for usein diagnostic procedures.

HardwareProduct Description Customer NumberLuminex® FLEXMAP 3D® with xPONENT FLEXMAP-3D-RUOHeater Block for 96-well microtiter plates CN-0224-01Heater

SoftwareProduct Description Customer NumberLuminex xPONENT Software for FLEXMAP 3D®CN-SW16-01ReagentsProduct Description Customer NumberxMAP Sheath Fl

NOTE: The Luminex® FLEXMAP 3D® Probe Height Adjustment Tool is to beused for 384 well mylar bottom and 384 well filter bottom plates only.To calibrate

2. Click Eject from the System Status bar to eject the XY platform.3.Place the probe height adjustment tool on the XY platform.4. From the Home page c

For research use only. Not for use indiagnostic procedures.FLEXMAP 3D® Probe Height Adjustment Tool69

Chapter 3 Maintenance and Cleaning ...27General Maintenance Precautions ...

Luminex® FLEXMAP 3D® Hardware User Manual70For research use only. Not for usein diagnostic procedures.

Short Term Maintenance ScheduleAppendix E: Maintenance Schedule 71

Luminex® FLEXMAP 3D® Hardware User Manual72For research use only. Not for use in diagnostic procedures.

Long Term Maintenance ScheduleAppendix E: Maintenance Schedule 73

Luminex® FLEXMAP 3D® Hardware User Manual74For research use only. Not for use in diagnostic procedures.

Appendix E: Maintenance Schedule 75

Luminex® FLEXMAP 3D® Hardware User Manual76For research use only. Not for usein diagnostic procedures.

Appendix D FLEXMAP 3D® Probe Height Adjustment Tool ...........67Appendix E Maintenance Schedule ......